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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
Fecha : |
02/10/2014 |
Actualizado : |
22/10/2019 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
AYALA, W.; JAURENA, M.; DALL AGNOLL, M.; CONDON, F.; BERRETTA, E.J. |
Afiliación : |
WALTER AYALA SILVERA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARTIN ALEJANDRO JAURENA BARRIOS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FEDERICO CONDON PRIANO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ELBIO JOAQUIN BERRETTA CARVALLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Rangeland germplasm resources: The case of Campos region in South America |
Fecha de publicación : |
2011 |
Fuente / Imprenta : |
In: Feldman, S.R.; Oliva, G.E.; Sacido, M.B. (eds.). International Rangeland Congress : diverse rangelands for sustainable society, (9º, 2011, Rosario, Argentina). INTA. 8 p. |
Idioma : |
Inglés |
Contenido : |
Native grasslands represent one of the largest natural ecosystems in the Southern Cone of South America and in the world. Around 70 million hectares in Central-East Argentina, Uruguay and Southern Brazil are the main support of livestock production. Mainly beef and sheep production are the activities of high economical relevance in the region with human and social implications. Its relevance is identified at regional level and also in the International Agenda. The joint IGC and IRC in 2008 remarked the importance of natural grasslands areas to provide environmental and productive solutions to demands of a growing world population. However, natural resources and traditional production systems are under extreme degradation/reduction as a consequence of intensification of the agriculture and forestry that are competing for land use in the region , with a significant reduction of biodiversity of genetic resources. |
Palabras claves : |
CAMPOS REGION; GERMPLASM RESOURCES; GRASSES; LEGUMES; NATIVE GRASSLAND. |
Thesagro : |
GERMOPLASMA; GRAMINEAS; LEGUMINOSAS; PASTIZAL NATURAL; PASTURAS; PASTURAS NATURALES. |
Asunto categoría : |
F03 Producción y tratamiento de semillas |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3414/1/Ayala-2011.pdf
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Marc : |
LEADER 01839nam a2200289 a 4500 001 1050882 005 2019-10-22 008 2011 bl uuuu u01u1 u #d 100 1 $aAYALA, W. 245 $aRangeland germplasm resources$bThe case of Campos region in South America$h[electronic resource] 260 $aIn: Feldman, S.R.; Oliva, G.E.; Sacido, M.B. (eds.). International Rangeland Congress : diverse rangelands for sustainable society, (9º, 2011, Rosario, Argentina). INTA. 8 p.$c2011 520 $aNative grasslands represent one of the largest natural ecosystems in the Southern Cone of South America and in the world. Around 70 million hectares in Central-East Argentina, Uruguay and Southern Brazil are the main support of livestock production. Mainly beef and sheep production are the activities of high economical relevance in the region with human and social implications. Its relevance is identified at regional level and also in the International Agenda. The joint IGC and IRC in 2008 remarked the importance of natural grasslands areas to provide environmental and productive solutions to demands of a growing world population. However, natural resources and traditional production systems are under extreme degradation/reduction as a consequence of intensification of the agriculture and forestry that are competing for land use in the region , with a significant reduction of biodiversity of genetic resources. 650 $aGERMOPLASMA 650 $aGRAMINEAS 650 $aLEGUMINOSAS 650 $aPASTIZAL NATURAL 650 $aPASTURAS 650 $aPASTURAS NATURALES 653 $aCAMPOS REGION 653 $aGERMPLASM RESOURCES 653 $aGRASSES 653 $aLEGUMES 653 $aNATIVE GRASSLAND 700 1 $aJAURENA, M. 700 1 $aDALL AGNOLL, M. 700 1 $aCONDON, F. 700 1 $aBERRETTA, E.J.
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INIA Tacuarembó (TBO) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
25/01/2022 |
Actualizado : |
25/01/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
LOUGE URIARTE, E.; GONZÁLEZ PASAYO, R.; MASSÓ, M.; CARRERA PAÉZ, L.; DOMÍNGUEZ MONCLA, M.; DONIS, N.; MALENA, R.; MÉNDEZ, A.; MORRELL, E.; GIANNITTI, F.; ARMENDANO, J.I.; FAVERIN, C.; CENTRÓN, D.; PARREÑO, V.; ODEÓN, A.C.; QUIROGA, M.P.; MOREIRA, A.R. |
Afiliación : |
ENRIQUE L. LOUGE URIARTE, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; RAMÓN A. GOZÁLEZ PASAYO, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; MARIANA MASSÓ, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; LAURA CARRERA PAÉZ, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; MANUEL DOMÍNGUEZ MONCLA, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; NICOLÁS DONIS, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; ROSANA MALENA, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; ALEJANDRA MÉNDEZ, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; ELEONORA MORRELL, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOAQUÍN I. ARMENDANO, Departamento de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Paraje Arroyo Seco s/n, Tandil, 7000, Argentina; CLAUDIA FAVERIN, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; DANIELA CENTRÓN, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; VIVIANA PARREÑO, Incuinta, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Virología e Innovaciones Tecnológicas, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IVIT, INTA-CONICET), Castelar, Buenos Aires, 1712, Argentina; ANSELMO C. ODEÓN, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; MARÍA PAULA QUIROGA, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; ANA RITA MOREIRA, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina. |
Título : |
Molecular characterization of multidrug-resistant Escherichia coli of the phylogroups A and C in dairy calves with meningitis and septicemia. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Microbial Pathogenesis, 2022, Volume 163, Article number 105378. doi: https://doi.org/10.1016/j.micpath.2021.105378 |
ISSN : |
0882-4010 |
DOI : |
10.1016/j.micpath.2021.105378 |
Idioma : |
Inglés |
Notas : |
Article history: Received 8 October 2021; Received in revised form 21 December 2021; Accepted 28 December 2021; Available online 1 January 2022.
Corresponding authors: Louge Uriarte, E.L.; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Ruta 226 km 73.5, Balcarce, Buenos Aires, Argentina; email:lougeuriarte.enrique@inta.gob.ar --- Quiroga, M.P.; Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina; email:paula.quiroga@conicet.gov.ar -- |
Contenido : |
ABSTRACT.- Escherichia coli is an important cause of septicemia (SEPEC) and neonatal meningitis (NMEC) in dairy calves. However, the diversity of virulence profiles, phylogroups, antimicrobial resistance patterns, carriage of integron structures, and fluoroquinolone (FQ) resistance mechanisms have not been fully investigated. Also, there is a paucity of knowledge about the virulence profiles and frequency of potential SEPEC in feces from calves with or without diarrhea. This study aimed to characterize the virulence potential, phylogroups, antimicrobial susceptibility, integron content, and FQ-resistance mechanisms in Escherichia coli isolated from calves with meningitis and septicemia. Additionally, the virulence genes (VGs) and profiles of E. coli isolated from diarrheic and non-diarrheic calves were compared between them and together with NMEC and SEPEC in order to identify shared profiles. Tissue and fluid samples from eight dairy calves with septicemia, four of which had concurrent meningitis, were processed for bacteriology and histopathology. Typing of VGs was assessed in 166 isolates from diverse samples of each calf. Selected isolates were evaluated for antimicrobial susceptibility by the disk diffusion test. Phylogroups, integron gene cassettes cartography, and FQ-resistance determinants were analyzed by PCR, sequencing, and bioinformatic tools. Furthermore, 109 fecal samples and 700 fecal isolates from dairy calves with or without diarrhea were evaluated to detect 19 VGs by uniplex PCR. Highly diverse VG profiles were characterized among NMEC and SEPEC isolates, but iucD was the predominant virulence marker. Histologic lesions in all calves supported their pathogenicity. Selected isolates mainly belonged to phylogroups A and C and showed multidrug resistance. Classic (dfrA17 and arr3-dfrA27) and complex (dfrA17-aadA5::ISCR1::blaCTX-M-2) class 1 integrons were identified. Target-site mutations in GyrA (S83L and D87N) and ParC (S80I) encoding genes were associated with FQ resistance. The VGs detected more frequently in fecal samples included f17G (50%), papC (30%), iucD (20%), clpG (19%), eae (16%), and afaE-8 (13%). Fecal isolates displaying the profiles of f17 or potential SEPEC were found in 25% of calves with and without diarrhea. The frequency of E. coli VGs and profiles did not differ between both groups (p > 0.05) and were identical or similar to those found in NMEC and SEPEC. Overall, multidrug-resistant E. coli isolates with diverse VG profiles and belonging to phylogroups A and C can be implicated in natural cases of meningitis and septicemia. Their resistance phenotypes can be partially explained by class 1 integron gene cassettes and target-site mutations in gyrA and parC. These results highlight the value of antimicrobial resistance surveillance in pathogenic bacteria isolated from food-producing animals. Besides, calves frequently shed potential SEPEC in their feces as commensals (?Trojan horse?). Thus, these bacteria may be disseminated in the farm environment, causing septicemia and meningitis under predisposing factors.
© 2022 Elsevier Ltd MenosABSTRACT.- Escherichia coli is an important cause of septicemia (SEPEC) and neonatal meningitis (NMEC) in dairy calves. However, the diversity of virulence profiles, phylogroups, antimicrobial resistance patterns, carriage of integron structures, and fluoroquinolone (FQ) resistance mechanisms have not been fully investigated. Also, there is a paucity of knowledge about the virulence profiles and frequency of potential SEPEC in feces from calves with or without diarrhea. This study aimed to characterize the virulence potential, phylogroups, antimicrobial susceptibility, integron content, and FQ-resistance mechanisms in Escherichia coli isolated from calves with meningitis and septicemia. Additionally, the virulence genes (VGs) and profiles of E. coli isolated from diarrheic and non-diarrheic calves were compared between them and together with NMEC and SEPEC in order to identify shared profiles. Tissue and fluid samples from eight dairy calves with septicemia, four of which had concurrent meningitis, were processed for bacteriology and histopathology. Typing of VGs was assessed in 166 isolates from diverse samples of each calf. Selected isolates were evaluated for antimicrobial susceptibility by the disk diffusion test. Phylogroups, integron gene cassettes cartography, and FQ-resistance determinants were analyzed by PCR, sequencing, and bioinformatic tools. Furthermore, 109 fecal samples and 700 fecal isolates from dairy calves with or without diarrhea were evaluated to detect... Presentar Todo |
Palabras claves : |
CALVES; E. COLI; Integrons; Multidrug resistance; Phylogroups; Quinolone resistance-determining region (QRDR) mutations; Virulence genes. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
Marc : |
LEADER 05268naa a2200433 a 4500 001 1062698 005 2022-01-25 008 2022 bl uuuu u00u1 u #d 022 $a0882-4010 024 7 $a10.1016/j.micpath.2021.105378$2DOI 100 1 $aLOUGE URIARTE, E. 245 $aMolecular characterization of multidrug-resistant Escherichia coli of the phylogroups A and C in dairy calves with meningitis and septicemia.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 8 October 2021; Received in revised form 21 December 2021; Accepted 28 December 2021; Available online 1 January 2022. Corresponding authors: Louge Uriarte, E.L.; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Ruta 226 km 73.5, Balcarce, Buenos Aires, Argentina; email:lougeuriarte.enrique@inta.gob.ar --- Quiroga, M.P.; Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina; email:paula.quiroga@conicet.gov.ar -- 520 $aABSTRACT.- Escherichia coli is an important cause of septicemia (SEPEC) and neonatal meningitis (NMEC) in dairy calves. However, the diversity of virulence profiles, phylogroups, antimicrobial resistance patterns, carriage of integron structures, and fluoroquinolone (FQ) resistance mechanisms have not been fully investigated. Also, there is a paucity of knowledge about the virulence profiles and frequency of potential SEPEC in feces from calves with or without diarrhea. This study aimed to characterize the virulence potential, phylogroups, antimicrobial susceptibility, integron content, and FQ-resistance mechanisms in Escherichia coli isolated from calves with meningitis and septicemia. Additionally, the virulence genes (VGs) and profiles of E. coli isolated from diarrheic and non-diarrheic calves were compared between them and together with NMEC and SEPEC in order to identify shared profiles. Tissue and fluid samples from eight dairy calves with septicemia, four of which had concurrent meningitis, were processed for bacteriology and histopathology. Typing of VGs was assessed in 166 isolates from diverse samples of each calf. Selected isolates were evaluated for antimicrobial susceptibility by the disk diffusion test. Phylogroups, integron gene cassettes cartography, and FQ-resistance determinants were analyzed by PCR, sequencing, and bioinformatic tools. Furthermore, 109 fecal samples and 700 fecal isolates from dairy calves with or without diarrhea were evaluated to detect 19 VGs by uniplex PCR. Highly diverse VG profiles were characterized among NMEC and SEPEC isolates, but iucD was the predominant virulence marker. Histologic lesions in all calves supported their pathogenicity. Selected isolates mainly belonged to phylogroups A and C and showed multidrug resistance. Classic (dfrA17 and arr3-dfrA27) and complex (dfrA17-aadA5::ISCR1::blaCTX-M-2) class 1 integrons were identified. Target-site mutations in GyrA (S83L and D87N) and ParC (S80I) encoding genes were associated with FQ resistance. The VGs detected more frequently in fecal samples included f17G (50%), papC (30%), iucD (20%), clpG (19%), eae (16%), and afaE-8 (13%). Fecal isolates displaying the profiles of f17 or potential SEPEC were found in 25% of calves with and without diarrhea. The frequency of E. coli VGs and profiles did not differ between both groups (p > 0.05) and were identical or similar to those found in NMEC and SEPEC. Overall, multidrug-resistant E. coli isolates with diverse VG profiles and belonging to phylogroups A and C can be implicated in natural cases of meningitis and septicemia. Their resistance phenotypes can be partially explained by class 1 integron gene cassettes and target-site mutations in gyrA and parC. These results highlight the value of antimicrobial resistance surveillance in pathogenic bacteria isolated from food-producing animals. Besides, calves frequently shed potential SEPEC in their feces as commensals (?Trojan horse?). Thus, these bacteria may be disseminated in the farm environment, causing septicemia and meningitis under predisposing factors. © 2022 Elsevier Ltd 653 $aCALVES 653 $aE. COLI 653 $aIntegrons 653 $aMultidrug resistance 653 $aPhylogroups 653 $aQuinolone resistance-determining region (QRDR) mutations 653 $aVirulence genes 700 1 $aGONZÁLEZ PASAYO, R. 700 1 $aMASSÓ, M. 700 1 $aCARRERA PAÉZ, L. 700 1 $aDOMÍNGUEZ MONCLA, M. 700 1 $aDONIS, N. 700 1 $aMALENA, R. 700 1 $aMÉNDEZ, A. 700 1 $aMORRELL, E. 700 1 $aGIANNITTI, F. 700 1 $aARMENDANO, J.I. 700 1 $aFAVERIN, C. 700 1 $aCENTRÓN, D. 700 1 $aPARREÑO, V. 700 1 $aODEÓN, A.C. 700 1 $aQUIROGA, M.P. 700 1 $aMOREIRA, A.R. 773 $tMicrobial Pathogenesis, 2022, Volume 163, Article number 105378. doi: https://doi.org/10.1016/j.micpath.2021.105378
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